Figure 5From: Effect of substrate stiffness on early human embryonic stem cell differentiationAlkaline Phosphatase Activity for cells collected from various substrates. Cells collected from different substrates after 4 and 7Â days in culture were re-plated on feeders in the presence of complete hESC medium for four days, following which they were stained for AP activity. A: Cells from 10: 1 PDMS, B: Cells from 20: 1 PDMS, C: Cells from TCPS, D: Cells from MEF.Back to article page