Figure 4

Multipotent differentiation of endometrial stem cells. Endometrial stem cells were cultured under appropriate differentiation media as described in box1-15 and assessed for differentiation using the indicated staining methods. A) Adipocytic differentiation, the red color indicates lipid vacuoles stained by Oil-Red. B) Osteocytic differentiation, red indicates calcium stained by Alizarin Red and C) Von-Kossa staining of osteoclasts differentiated from EnSCs. D) Chondrocytic differentiation, the blue color resulted from Alcian blue staining. E) Neuronal differentiation, the red conjugated mAb indicates pre-neural marker Nestin stain. F) Angiogenesis of EnSCs, the green indicated CD31 stain. G) Oligodendrocytic differentiation, the red conjugated mAb indicates receptor interacting protein (RIP). H) Hepatocytic differentiation, green indicates albumin stain. I) Myocytic differentiation, red indicates α-actinin stain.