Figure 2
Optical temperature measurement in MTPs applying a fluorescent assay with the dyes Rhodamin B and Rhodamin 110. (A) Progress of the fluorescence signals of Rhodamin B (RhB) and Rhodamin 110 (Rh110) and their calculated ratio in a single well with varied thermostat set point temperature. Contrary to Figure 1C heating and cooling circulation systems were operated with one thermostat. (B) Calibration curve of the well temperature (measured via PT100 thermometer) vs. the fluorescence ratio RhB/Rh110. Experimental conditions: 96well MTP, VL = 200 μL, n = 995 rpm, d0 = 3 mm, RT = 37°C.