Figure 4
Cultivation of E.coli BL21 expressing the fluorescent protein FbFP applying a temperature profile in a MTP. (A) Cultivation and online monitoring of microbial growth (via scattered light) in complex TB medium without induction. (B) Cultivation and online monitoring of microbial growth and (C) fluorescent protein formation in complex auto-induction OnEx. Culture conditions: 96well MTP, VL = 200 μL, n = 995 rpm, d0 = 3 mm, aeration with 100% oxygen. Temperature profile: RT = 37°C, Tset,low = 5°C, Tset,high = 60°C (comp. Figure 3D). Data of 12 (from 48) exemplary wells.