Figure 4
Learning at various annealing temperatures for the protocol optimization: 25°C (lanes 1 and 2), 40°C (lanes 3 and 4), 55°C (lanes 5 and 6), 60°C (lanes 7 and 8), and 70°C (lanes 9 and 10). The odd-number lanes are for the negative controls with memory tags only and without input gDNA, and the even numbers are for the positive controls with both memory tags and input gDNA. The learning products were analyzed using the ethidium bromide staining 4-20% Urea-PAGE at 60°C. The contrasts of the top image for LPH and the bottom image for LPs were adjusted separately from the orginal Urea-PAGE image (see Additional file 2: Figure S2) to better assess the results.