Fig. 5

Online and offline data from parallel fermentations of E. coli BL21 EcFbFP under diauxic conditions. Cultivation in WilmsMOPS mineral medium supplemented with 20 g/L glucose and 1.5 g/L sorbitol, buffered with 0.2 M MOPS. Culture conditions for RAMOS: 250 mL RAMOS shake flasks, V_L = 12 mL, n = 350 rpm, d₀ = 50 mm, T = 30 °C. Culture conditions for RoboLector: 48-well Flowerplate, V_L = 0.6 mL, n = 1000 rpm, d₀ = 3 mm, T = 30 °C. Lines shown in (b) and (c) for OD₆₀₀, glucose, sorbitol, acetate and pH represent the averages of the offline data obtained from 250 mL shake flasks and 48-well microtiter plate at the same time points. For better visibility, the sorbitol concentrations were multiplied by a factor of five and the product fluorescence values by a factor of three. The k_La value used for calculation of the OTR_calc course was (a) 276 h⁻¹