Fig. 7

Online and offline data from parallel fermentations of G. oxydans 3504 in unbuffered and buffered complex media. Cultivation in unbuffered (a, c, e) and 100 mmol/L PIPPS buffered (b, d, f) complex medium containing 40 g/L glucose. Culture conditions for RAMOS: 250 mL RAMOS shake flasks, V_L = 10 mL, n = 350 rpm, d₀ = 50 mm, T = 30 °C. Culture conditions for BioLector: 48-well Flowerplates, V_L = 1 mL, n = 1250 rpm, d₀ = 3 mm, T = 30 °C. The concentration of the intermediates 2-ketogluconic acid (2-KGA) and 5-ketogluconic acid (5-KGA) were multiplied by a factor of five for better visibility. The k_La values used for calculation of the OTR_calc course for unbuffered and buffered conditions were 425 h⁻¹ and 413 h⁻¹, respectively