Fig. 3
From: Essential validation methods for E. coli strains created by chromosome engineering
Evaluation of E. coli strains based on cellular growth or morphology characteristics. a Growth curves of the AB1157 (black) and recombineered ΔmotAB cells (red) in shake flasks containing LB medium at 37 °C and 250 rpm. b A simple method using semi-log plot to find the log phase of the growth curve for AB1157 (black) and ΔmotAB (red) strains. The linear region of the semilog plot is the log phase of the growth curve. c The exponential fitting of the selected log phase of the growth curve for AB1157 (black) and ΔmotAB (red) strains. From the fit (dotted lines), the growth rates (μ) are determined as 1.11 h−1 and 1.13 h−1 for one sample of AB1157 and ΔmotAB strains repectively. d A sample phase contrast image of AB1157 cells which were grown in LB medium at 37 °C and 250 rpm is shown. Such images were analyzed by MicrobeTracker software to calculate precisely the cell length and volume for each cell. e & f The data of cell length and cell volume of ~350 cells for each strain are plotted using a Box and Whiskers plot. The line within the box corresponds to the median value, the borders show the upper and lower quartiles (75 % and 25 %), and the whiskers represent the maximum and minimum values