Fig. 2

Orthogonality tests performed on a simple combination of two Biobrick parts. a Fluorescence output displayed by E. coli XL1 strain transformed with a single plasmid containing a constitutive promoter coupled to a green fluorescent protein (Bb1), a single plasmid containing the same promoter coupled to a red fluorescent protein (Bb2), and a combination of both plasmids. Plots showing flow cytometry measurements performed on individual cells (dots). b Comparison of the proteomic profile of an E. coli strain constitutively expressing a green fluorescent protein (green lines) with that of the same strain carrying an empty plasmid (red lines) and the control non-transformed strain (blue lines). Proteins showing a statistically significant change in expression are numbered according to Additional file 1: Table S2