Fig. 3

In vitro assays for cytotoxicity of GO and growth of C2C12 myoblasts on RGD/PLGA matrices. a C2C12 myoblast viability after the 24 h of incubation with GO. b Initial adhesion and (c) proliferation of C2C12 myoblasts cultured on the control (TCP), PLGA matrices and RGD/PLGA nanofiber matrices with or without the GO. The viability, initial adhesion and proliferation of C2C12 myoblasts were determined using a CCK-8 assay. An asterisk (*) denotes a significant difference between the control and other groups, p < 0.05. The data is expressed as mean ± SD based on at least duplicate observations from three independent experiments