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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Heterologous expression of the isopimaric acid pathway in Nicotiana benthamiana and the effect of N-terminal modifications of the involved cytochrome P450 enzyme

Fig. 2

Metabolite analysis of infiltrated plant leaves. a Total Ion Chromatograms (TIC) for the detection of isopimaradiene using GC-MS analysis. Samples were spiked with the internal standard Eicosene (ISTD). b Mass spectra of isopimaradiene detected in the standard and diTPS-ISO infiltrated leaf extracts. c Extracted ion chromatograms (m/z −303[M + H]+, 325[M + Na]+, 347 [M + 2Na]+) for the detection of isopimaric acid using LC-MS analysis. Isopimaric acid is detected at retention time 10 min. d Mass spectra of isopimaric acid detected in the standard and CYP720B4 + diTPS-ISO infiltrated leaf extracts

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Journal of Biological Engineering

ISSN: 1754-1611