Fig. 4

CYP720B4 modifications used in this work. a Schematic representation of CYP720B4 sequences, where the catalytic part of the protein sequence is depicted in green, while the N-terminal parts are noted in more detail: Tm domain = Transmembrane domain of the CYP720B4;  Tp  - Transit peptide (the N-terminal 52 amino acid of the Arabidopsis ferredoxin protein FedA); and Barnes modification  - omission the first 21 bases of the coding sequence and introduction of three point mutations in the N-terminal part. b Protein sequence alignment of the N-terminal parts of CYP720B4, Barnes-CYP720B4 and ΔTm-CYP720B4. The point mutations of Barnes-CYP are highlighted in yellow