Table 6 DNA primers used to amplify yeast promoters
From: End-to-end automated microfluidic platform for synthetic biology: from design to functional analysis
Pgal1-250Â F | GGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCACGAATCAAATTAACAACCA |
Pgal1-100Â F | GGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCATTTTCAGTTTGTATTACTT |
Pgal1-R | TTGGGACAACACCAGTGAATAATTCTTCACCTTTAGACATTATAGTTTTTTCTCCTTGAC |
Pleu2-250Â F | GGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCGCATATACCTTTTTCAACTG |
Pleu2-100Â F | GGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTTTTCCAATAGGTGGTTAGC |
Pleu2-R | TTGGGACAACACCAGTGAATAATTCTTCACCTTTAGACATTAGAATGGTATATCCTTGAA |
Pspo13-250Â F | GGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTATTTACACATCTAATTTTT |
Pspo13-100Â F | GGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCAAATAGCCGCCGACAAAAAG |
Pspo13-R | TTGGGACAACACCAGTGAATAATTCTTCACCTTTAGACATAATTATTCTCGACTCAACTT |
Ptef1-250Â F | GGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCAAAAGAGACCGCCTCGTTTC |
Ptef1-100Â F | GGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCAAGTTTCAGTTTCATTTT |
Ptef1-R | TTGGGACAACACCAGTGAATAATTCTTCACCTTTAGACATTTTGTAATTAAAACTTAGAT |