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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform

Fig. 2

a Analysis of eGFP expression. a SDS-PAGE and b Western Blot and Ponceau S staining as loading control of total cell extracts. The positive control (Lys-eGFP) was diluted 1/10 for Western blot analysis to prevent overexposure. c SDS-PAGE of purified Plk-eGFP and Alk-eGFP in comparision to wild-typ Lys-eGFP. b normalized fluorescence spectra of Lys-eGFP, Plk-eGFP and Alk-eGFP. ESI-MS/MS spectra of elastase digested Plk-eGFP (c) and Alk-eGFP (d), respectively, with assigenment of the fragments to the uAA-eGFP amino acid sequence

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Journal of Biological Engineering

ISSN: 1754-1611