Fig. 3

Correction for flavin autofluorescence. a Raw eGFP fluorescence intensity (λ_Ex = 475 nm, λ_Em = 507 nm) of non-induced cultures (grey) and induced cultures supplemented with 433 μM IPTG and 38 mM Plk (red). b Flavin fluorescence intensity (left axis, squares, λ_Ex = 450 nm, λ_Em = 528 nm) and scattered light intensity (right axis, circles, λ_Ex = 650 nm, λ_Em = 650 nm). Only every 5^th data point is shown to improve readability. c Corrected eGFP signal (I_{eGFP,corrected} = I_eGFP,raw – 0.47 I_flavin) as applied in subsequent figures. Error bars indicate standard deviation of triplicates