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Fig. 5 | Journal of Biological Engineering

Fig. 5

From: Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform

Fig. 5

Response surface model for Plk-eGFP production. eGFP fluorescence of E. coli BL21(DE3) uAA-eGFP cultures after 24 h as predicted by the response surface model as a function of Plk concentration, IPTG concentration and time of IPTG addition (tplk = 0 h). Dark red colors indicate operating conditions which lead to high Plk-eGFP fluorescence

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