Fig. 5From: Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platformResponse surface model for Plk-eGFP production. eGFP fluorescence of E. coli BL21(DE3) uAA-eGFP cultures after 24 h as predicted by the response surface model as a function of Plk concentration, IPTG concentration and time of IPTG addition (tplk = 0 h). Dark red colors indicate operating conditions which lead to high Plk-eGFP fluorescenceBack to article page