Design and engineering of a transmissible antiviral defense

Table 3 Population-level vaccine protection against lethal virus

Lag between vaccine and lethal virus addition ^a	Fold drop in concentration/titer of vaccine-infected cells due to T5 killing ^b
no vaccine	4500, 15550
3 hr	1692
7 hr	283
overnight	4, 37

^aCells at 10⁸/mL were infected with vaccine and helper; the vaccine multiplicity of infection was 1, so infections by vaccine would thus have been nearly complete within 30 minutes
^bCounts to establish the baseline, before T5 addition, were of vaccine-infected (ampicillin-resistant) cells without specific regard to T5 sensitivity. (For ’no vaccine,’ initial counts are based on total cells.) At 3-4 hr after T5 addition (at the time of plating), T5 density was at least 10X that of cell density, so nearly all sensitive cells would have been been unable to form a colony

ISSN: 1754-1611