Fig. 5
From: Respiration activity monitoring system for any individual well of a 48-well microtiter plate
Characteristic segment of a measured single well oxygen partial pressure propagation during cultivation of H. polymorpha RB11 pC10-FMD (PFMD-GFP). (I) Phase of standard aeration with air (flow phase), 0.8 vvm, 16 min. (II) Phase of closed inlet and outlet valves and determination of the OTR (stop phase), 0 vvm, 3 min. (III) Phase of elevated aeration with supply air to compensate for the prior interruption of air supply (4.8 vvm, high flow phase). The presented oxygen partial pressure decrease during phase (II) corresponds to an OTR of 21.5 mmol L-1 h-1. Due to the dynamic equilibrium of oxygen supply and consumption of oxygen, the oxygen partial pressure during phase (I) is reduced and roughly 193 hPa (instead of 209.5 hPa for pure air)