Fig. 4
From: Can terminators be used as insulators into yeast synthetic gene circuits?
Mutational study on DEG1t-pCYCnoTATA and Tsynth8-pCYC1noTATA. Mutations on the efficiency element of DEG1t (mut_DEG1t-pCYC1noTATA) and Tsynth8 (mut_Tsynth8-pCYC1noTATA) make it no longer recognized by RNA polymerase II molecules as a TATA-box with a consequent decrease in fluorescent expression. The insertion of a 100-nucleotide-long spacer between Tsynth8 and pCYC1noTATA extends the distance between the TATA-box-like efficiency element and the TSS up to 157 nucletides. This prevent any TSS activation from the TATAAA motifs along Tsynth8. Fluorescence level are normalized to the one corresponding to pCYC1noTATA. The ∙ symbol indicates no statistically significant difference with respect to pCYC1noTATA fluorescence level, whereas the ∘ symbol indicates no statistically significant difference with respect to shortADH1t-pCYC1noTATA fluorescence level