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Fig. 9 | Journal of Biological Engineering

Fig. 9

From: Can terminators be used as insulators into yeast synthetic gene circuits?

Fig. 9

DEG1t as insulator of five different yeast constitutive promoters. DEG1t drives a decrease in the fluorescence level of strong and medium-strength promoters, whereas it seems not to spoil the transcriptional activity of the rather weak ACT1 promoter. Values shown in this Figure are the ratio between the fluorescence levels of the insulated promoters with respect to the ones of the non-insulated promoters. The ** symbol indicates a statistically significant difference between the fluorescence expressed by an insulated promoter with respect to the one corresponding to its wild-type configuration

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