Fig. 3

Extraction of the fluorescent model protein FbFP from E. coli BL21(DE3) pRhotHi-2 comparing the standard and HT protocol (according to Fig. 2a). a Fluorescence measurement of different fractions occurring during the extraction procedure applying the BioLector technique. Error bars indicate standard deviation of three simultaneously performed extraction experiments. b SDS-PAGE analysis of different fractions occurring during the extraction procedure. Target protein FbFP framed. Conditions: Cultivation in 10 mL OnEx auto-induction medium, 250 ml shake flask, n = 350 rpm, d₀ = 50 mm, 37 °C. Extraction with BugBuster 10× Protein Extraction Reagent