Fig. 6
Expression study of K. lactis expressing recombinant endoglucanase cel5A from Trichoderma reesei at varied medium composition applying the RoboLector system. Cultivation and on-line monitoring of microbial growth (via scattered light, a) and DOT (b) at varied galactose concentrations. The data were derived from representative single experiments. They are in very good agreement with preliminary, non-automated characterization experiments focusing on growth kinetics and final product yield (data not shown). c Automated offline analysis of volumetric cellulase activity applying the AzoCMC assay. Error bars indicate standard deviation of three simultaneously performed assays. Cultivation conditions: 48well Flower plate, YP medium containing galactose as carbon source, VL = 1 ml, n = 1500 rpm, d0 = 3 mm, 30 °C. Activity assay conditions: incubation in 96well DWP, 0.2 g L−1 Azo-CMC in 0.1 M acetate buffer (pH = 4.8), VL = 400 μL, n = 900 rpm, d0 = 3 mm, 50 °C, absorbance measurement at 590 nm