Fig. 2
From: Construction of an easy-to-use CRISPR-Cas9 system by patching a newly designed EXIT circuit
Validation of the control module of the EXT circuit. a Schematic of the control module of the EXIT circuit constructed in E. coli. b Characterization of I-SceI expression from the I-SceI-GFPmut3b pEC001 plasmid in response to different concentrations of the L-arabinose inducer. c-d Evaluation of control module responses to different L-arabinose concentrations at the population level (c) and at the single-cell level (d). e Elimination efficiencies of the control module at different induction times. f Visual demonstration of elimination efficiency on plates. Symbols in Panel c: (■) No inducer and (●) 1 mM; (▲) 5 mM; (▼) 10 mM; (♦) 100 mM L-arabinose. For panels c and e, three samples were evaluated and the standard errors are indicated