Study of in vitro transcriptional binding effects and noise using constitutive promoters combined with UP element sequences in Escherichia coli

Table 1 An electrophoretic mobility shift assay (EMSA) was used to calculate dissociation/association constant for various strength core promoter constructs, half UP element-core promoter constructs, and UP element constructs-core promoter

	RFU	B_max	K_d (nM)	CV	R²
J23119	2038.92 ± 104.29	0.99	37.78	68.0 ± 2.98	0.90
J23119 + 1/2UP	44.7 ± 9.41	0.99	21.03	477.8 ± 63.27	0.90
J23119 + UP	2726.88 ± 53.52	0.99	42.02	72.6 ± 1.40	0.90
J23102	86.67 ± 5.87	0.98	47.38	145.09 ± 7.95	0.90
J23102 + 1/2UP	85.37 ± 6.03	0.99	45.51	148.90 ± 9.42	0.90
J23102 + UP	705 ± 4.86	0.99	44.82	92.25 ± 38.40	0.90
J23108	23.31 ± 1.49	0.99	50.06	72.6 ± 7.40	0.92
J23108 + 1/2 UP	18.2 ± 0.30	0.26	21.97	69.1 ± 2.84	0.96
J23108 + UP	1042.43 ± 12.12	0.99	31.43	57.2 ± 0.91	0.90
J23115	10.42 ± 1.26	0.99	55.00	93.3 ± 45.08	0.90
J23115 + 1/2 UP	12.8 ± 0.28	0.16	51.71	71.3 ± 8.96	0.90
J23115 + UP	994.27 ± 13.14	0.99	38.14	64.54 ± 5.71	0.90
J23112	1.30 ± 0.040	0.15	50.8	321.96 ± 21.96	0.90
J23112 + 1/2UP	4.96 ± 0.10	0.64	68.16	440.39 ± 19.24	0.91
J23112 + UP	1.44 ± 0.11	0.56	12.55	296.93 ± 24.64	0.90
J23109	1 ± 0.005	0.16	46.05	258.17 ± 111.29	0.90
J23109 + 1/2UP	9.93 ± 0.66	0.66	64.75	171.87 ± 31.28	0.90
J23109 + UP	5.68 ± 0.99	0.50	7.44	158.75 ± 28.40	0.93

ISSN: 1754-1611