Fig. 1
From: Viral Cre-LoxP tools aid genome engineering in mammalian cells
Schematic illustration of design and construction of recombinant AAV and lentiviral vectors for Cre expression. Features of lentiviral vectors, depicting the humanized Cre gene under the control of the CMV promoter without a selection marker (a) or in a combination with optional selection markers, including GFP (b), Puro (c), GFP plus Puro (d). The Cumate-inducible expression of CRE using an all-in-one lentiviral vector (e). Similarly, features of the AAV vectors, depicting the humanized Cre gene under the control of the CMV promoter alone (f) for with optional selection markers GFP (g), Puro (h), or GFP plus Puro (i)