Fig. 6

Analysis via NBM of the measured HSL-dependent output in the re-designed circuit variants, without Monitor cassette. a Circuit description. All of them are available with an RFP expression system downstream of the output promoter (indicated in the text with the r suffix). Symbols are described in Figure 1, except white ovals that represent a weak RBS (BBa_B0033), used in these circuits to decrease tetR expression. b Fitting of the training set circuit X₂T_wr. Estimated parameters were: α_T = 3.03 (4%), K_T = 63.14 (17%), η_T = 0.99 (10%), δ_T = 0.02 (>100%), where symbols have the same meaning as in Table 1 and CVs are reported in brackets. c-d) Prediction of the test set circuit X₁T_wLr and X_repT_wLr, respectively. In panels B-D, circles represent the average measured value and error bars represent the 95% confidence intervals of the mean. Solid line represents the median output of the model calculated via Monte Carlo simulations for each HSL concentration tested. Dashed dark red lines are the 95% confidence bands of the output distribution. Dashed light red lines are the 95% confidence bands of the output distribution calculated after multivariate sensitivity analysis