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Table 1 Steady-state kinetic parameters of Cupriavidus taiwanensis tryptophan hydroxylase W192F (CtAAAH-F) and variants produced by semi-rational evolution

From: Synthetic pathways and processes for effective production of 5-hydroxytryptophan and serotonin from glucose in Escherichia coli

 

Km (mM)

kcat (s−1)

Vmax (mM · s−1)

Relative kcat/Km

CtAAAAH-F

3.0

0.4

1.2

0.13

CtAAAAH-F197I

2.1

1.3

2.7

0.62

CtAAAAH-F197 L

1.6

1.1

1.8

0.69

CtAAAAH-E219C

1.8

1.5

2.7

0.83

CtAAAH-LC

0.95

1.9

1.8

2.00

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Journal of Biological Engineering

ISSN: 1754-1611