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Table 1 Steady-state kinetic parameters of Cupriavidus taiwanensis tryptophan hydroxylase W192F (CtAAAH-F) and variants produced by semi-rational evolution

From: Synthetic pathways and processes for effective production of 5-hydroxytryptophan and serotonin from glucose in Escherichia coli

  Km (mM) kcat (s−1) Vmax (mM · s−1) Relative kcat/Km
CtAAAAH-F 3.0 0.4 1.2 0.13
CtAAAAH-F197I 2.1 1.3 2.7 0.62
CtAAAAH-F197 L 1.6 1.1 1.8 0.69
CtAAAAH-E219C 1.8 1.5 2.7 0.83
CtAAAH-LC 0.95 1.9 1.8 2.00