Fig. 2

Decellularization steps: (a) Decellularization of the pancreatic body tail scaffold. Images illustrate the gradual change in color caused by perfusion-decellularization in mouse pancreases. Treatment with 1% triton x-100 resulted in a decellularized pancreas after 315 min. b H&E staining showing that there were no remnant cells after the completion of decellularization. c DNA quantification demonstrating that the amount of DNA was clearly lower in the treated organs than in natural organs (from 931.9 ± 267.8 to 11.7 ± 3.6 ng/mg), P < 0.05