Fig. 9

Glucose-stimulated insulin secretion assay: (a) There was no significant difference in the amount of secreted insulin at basal conditions (2 mM glucose) or after stimulation with 20 mM glucose between the control and perfusion-culture groups. b Insulin secretion is presented as a % content, and the results showed that insulin levels were higher in animals stimulated with 20 mM glucose than at basal conditions (2 mM glucose) in both the control group and the perfusion-culture group. The error bars show the SEMs, n = 6. ****, P < 0.01. The amount of secreted insulin was normalized against total protein levels