Table 3 List of strains and plasmids used in this work

Strains

Genotype

Source

 S. roseiscleroticus

Wild type

ATCC

 S. lividans K4–114

pro-2 str-6SLP2 − SLP3 − act::ermE Streptomyces

[24]

 SR1

S. roseiscleroticus/pZJ183 (srcmRI overexpression)

This Work

 SR2

S. roseiscleroticus/pZJ196 (srcmRII disruption)

This Work

 SR3

S. roseiscleroticus/pZJ183 + pZJ196 (srcmRI overexpression and srcmRII disruption)

This Work

 KS1

S. lividans K4–114/pSUN6

This Work

Plasmids

Description

Source

 pZJ172

srcmS in pJET1.2

This Work

 pZJ178

srcmPKS in pJET1.2

This Work

 pZJ179

srcmRI in pJET1.2

This Work

 pZJ180

rcmPK in pJET1.2

This Work

 pZJ178

srcmPKS in pJET1.2

This Work

 pZJ179

srcmRI in pJET1.2

This Work

 pSUN6

srcmPKS in pRM5

This Work

 pTZ3

oxyABC in pRM5

This Work

 pZJ183

srcmRI in pSET152

This Work

 pZJ191

srcmRII knockout left arm in pJET1.2

This Work

 pZJ192

srcmRII knockout right arm in pJET1.2

This Work

 pZJ195

srcmRII knockout left arm in pKC1139

This Work

 pZJ196

srcmRII knockout left arm-right arm in pKC1139

This Work