Fig. 8

MCF-7 and MDA-MB-231 cells exhibit differential gene and protein expression profiles in response to CoCl₂ treatment. a Fold change in mRNA expression of CDKN1A, CDKN1B, CDK2, CDK4, CCNA2, CCND1, CCNE1, and MYC by qRT-PCR after 72 h of CoCl₂ treatment relative to untreated control (^* P < 0.05 compared to untreated control). b Western blot analysis of HIF1α, p21, p27 and β-actin (control) expression in MCF-7 and MDA-MB-231 cells after 72 h of CoCl₂ treatment in 2D and 3D (pHEMA-coated plate) cultures compared to untreated control. c Relative protein expression of p21 normalized to β-actin, with results represented as mean ± SD of three independent experiments (^* P < 0.05 compared to untreated control)