Table 1 Variables and their levels used in Plackett-Burman design for screening of culture conditions affecting the yield of plasmid DNA vaccine pcDNA-CCOL2A1 by the engineered E. coli DH5α
Symbol code | Factors | Experimental values | |
|---|---|---|---|
Low level (− 1) | High level (+1) | ||
A(g/L) | Peptone | 16.00 | 24.00 |
B(g/L) | Yeast extract | 10.00 | 15.00 |
C(g/L) | NaCl | 5.00 | 7.50 |
D(g/L) | Ampicilin | 0.05 | 0.10 |
E(mL/L) | Microelement | 10.00 | 15.00 |
F(g/L) | Mannitol | 5.00 | 7.50 |
G | Initial pH | 6.00 | 8.00 |
H(RPM) | Rotational speed | 180 | 250 |
J(°C) | Temperature | 30.00 | 37.00 |
K(OD) | Inoculum concentration | 0.10 | 0.20 |
L | Dummy factors | Â | Â |