Table 2 Plackett–Burman design matrix with response value for screening of culture conditions affecting the yield of plasmid DNA vaccine pcDNA-CCOL2A1 by the engineered E. coli DH5α
Run | Variable levels | Plasmid yield | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
A | B | C | D | E | F | G | H | J | K | L | (mg/L) | |
1 | 1 | −1 | 1 | 1 | −1 | 1 | 1 | 1 | − 1 | −1 | − 1 | 258.29 ± 6.44 |
2 | −1 | 1 | 1 | −1 | 1 | 1 | 1 | − 1 | − 1 | − 1 | 1 | 202.98 ± 7.37 |
3 | 1 | −1 | − 1 | − 1 | 1 | − 1 | 1 | 1 | − 1 | 1 | 1 | 267.36 ± 11.00 |
4 | −1 | − 1 | − 1 | −1 | − 1 | −1 | − 1 | −1 | − 1 | −1 | − 1 | 158.51 ± 11.30 |
5 | −1 | −1 | − 1 | 1 | − 1 | 1 | 1 | −1 | 1 | 1 | 1 | 234.83 ± 14.17 |
6 | 1 | 1 | 1 | −1 | −1 | −1 | 1 | −1 | 1 | 1 | −1 | 279.81 ± 10.27 |
7 | 1 | 1 | −1 | 1 | 1 | 1 | −1 | − 1 | − 1 | 1 | − 1 | 312.86 ± 13.69 |
8 | −1 | 1 | 1 | 1 | −1 | − 1 | −1 | 1 | − 1 | 1 | 1 | 198.70 ± 11.36 |
9 | −1 | −1 | 1 | −1 | 1 | 1 | −1 | 1 | 1 | 1 | −1 | 220.79 ± 8.51 |
10 | 1 | 1 | −1 | −1 | − 1 | 1 | − 1 | 1 | 1 | −1 | 1 | 284.63 ± 10.09 |
11 | −1 | 1 | −1 | 1 | 1 | −1 | 1 | 1 | 1 | −1 | − 1 | 146.60 ± 15.25 |
12 | 1 | −1 | 1 | 1 | 1 | −1 | − 1 | −1 | 1 | −1 | 1 | 181.27 ± 10.67 |