Table 4 Steepest ascent experiments to move the experimental region towards the maximum yield of plasmid DNA vaccine pcDNA-CCOL2A1 by the engineered E. coli DH5α
Run | Peptone(g/L) | Mannitol (g/L) | Inoculum concentration (OD) | Plasmid yield (mg/L) |
|---|---|---|---|---|
1 | 22 | 6 | 0.15 | 296.92 ± 10.00 |
2 | 24 | 7 | 0.25 | 313.76 ± 8.01 |
3 | 26 | 8 | 0.35 | 338.57 ± 11.92 |
4 | 28 | 9 | 0.45 | 326.04 ± 8.65 |
5 | 30 | 10 | 0.55 | 303.95 ± 8.46 |