Fig. 2

a Absolute fluorescence of constructs denoted by BioBrick ID (Additional File 2: Table S1), with (blue) and without (black) RiboJ insulation as measured by calibrated flow cytometry. Each dot represents the geometric mean fluorescence of n > 10,000 cells. b Fold change in fluorescence of constructs when insulated with RiboJ. Bars represent the fold change in the mean fluorescence across replicates, and dots represent all pairwise fold changes between replicates. The dashed line and grey region indicate one geometric SD factor around the geometric mean of a null fold change distribution computed from the fluorescence data