Fig. 4
From: The genetic insulator RiboJ increases expression of insulated genes
RiboJ increases gene expression levels. To characterize the impact of insulation with RiboJ on gene expression, a library of 24 frequently employed constitutive promoters was used to drive the expression of a sfGFP reporter construct. For each of the 24 promoters, we assembled two sets of reporter constructs that differed only by the presence RiboJ. Insulation with RiboJ increased mRNA expression by an average of twofold and increased protein expression between twofold and tenfold