Fig. 1

Inactivation of SACE_5754 in S. erythraea A226. a Genetic organization of SACE_5754 and its adjacent genes in S. erythraea A226. b Schematic deletion of SACE_5754 by linearized fragment homologous recombination in S. erythraea A226. c PCR confirmation of the SACE_5754 deletion mutant. Lanes: M, 5000-bp DNA ladder; pUCTSR∆5754, the positive control, 1727 bp amplified from pUCTSR∆5754; A226, the negative control, 624 bp amplified from A226; ∆SACE_5754, 1727 bp amplified from ∆SACE_5754. d. Er-A production in S. erythraea A226 and its derivatives. Mean values of three replicate are shown, with the standard deviation indicated by error bars. *p < 0.05, **p < 0.01, ***p < 0.001, ns, no significant (relative to A226)