Fig. 8
High release fed-batch cultivation of Escherichia coli BL21 (DE3) pRhotHi-2-EcFbFP in Wilms-MOPS medium. Data correspond to 96-square-well conventional batch microtiter plate (black, open symbols) and to the newly developed prototype 48-round-well fed-batch microtiter plate for high glucose release (blue, half-filled symbols). All offline measured data points are mean values of measurements of four individual wells, the error bars indicate the standard deviation. Exception: Oxygen transfer rate (OTR) and pH are measured in duplicates. A) Oxygen transfer rate (OTR); the OTR in the newly developed prototype microtiter plate is measured in six individual wells. The blueish shadow indicates the standard deviation of these measurements. B) Cell dry weight (CDW, squares) and pH (stars); C) Flavin mononucleotide binding fluorescent protein (FbFP, triangle) and FbFP-Yield per glucose (cross); D) Measured glucose (circle) and acetate (diamond) concentration. Dotted lines represent the calculated total glucose concentration available for the microorganisms until the respective point of cultivation. Cultivation conditions: initial biomass concentration: 0.11 g/L, temperature = 37 °C; pH0 = 7.5; shaking frequency: n = 970 rpm; shaking diameter: d = 3 mm; culture volume VL,96 = 600 μL/well, VL,48 = 800 μL/well; initial glucose concentration cS_Batch = 20 g/L, cS_FedBatch = 0 g/L. Glucose was used as sole carbon source