Fig. 2

The krCRISPR system enables efficient gene knockout for the PARP1 gene. a Schematic of the experimental workflow. b Representative gel pictures of T7E1 assay for detection of indels at PARP1 sites in HEK293T cells. The indel frequency was labeled below. Ctr is the PCR band from unmodified cells with T7 enzyme digestion. c Quantification for the T7E1 assay for Fig. 2b (n = 3, error bars showed mean ± SEM). d Examples of indel sequences for four single cell-derived clones. Schematic of the gRNA target site was shown above. PAM sequence is marked in orange. Cas9 cutting site is indicated by red arrow. Insertions are indicated by red letter