Fig. 2
Hydrolysates of CSN, CSN-Y270A, CSN-E59A, and CSN-W118A with chitosan polymer DA 1.5% as a substrate. Hydrolysates were produced by incubating 1 mg/ml chitosan polymer DA 1.5% and each enzyme (0.05 μM of CSN, 0.42 μM of CSN-Y270A, 0.05 μM of CSN-E59A, and 2.5 μM of CSN-W118A) for 16 h at 37 °C and then used for a TLC. A mixture of D1–6 was utilized as a standard