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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Identification of new glutamate decarboxylases from Streptomyces for efficient production of γ-aminobutyric acid in engineered Escherichia coli

Fig. 3

SDS-PAGE analysis of expression and purification of GADs from E. coli BL21(DE3). a Expression of GadB, StGAD, ScGAD, and SsGAD in E. coli BL21(DE3). M: protein ladder; 1 and 2: insoluble and soluble fractions of E. coli BL21(DE3)/pET28a (vector control); 3 and 4: soluble and insoluble fractions of E. coli BL21(DE3)/GadB; 5 and 6: soluble and insoluble fractions of E. coli BL21(DE3)/StGAD; 7 and 8: soluble and insoluble fractions of E. coli BL21(DE3)/SsGAD; 9 and 10: soluble and insoluble fractions of E. coli BL21(DE3)/ScGAD. b GADs purified from the engineered E. coli BL21(DE3) strains. 1: GadB; 2: StGAD; 3: SsGAD; 4: ScGAD. StGAD, SsGAD and GadB were expressed in E. coli BL21(DE3) at 28 °C, while ScGAD was expressed at 18 °C. The target band of the GADs are in the green box

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Journal of Biological Engineering

ISSN: 1754-1611