Systems metabolic engineering of Corynebacterium glutamicum for the bioproduction of biliverdin via protoporphyrin independent pathway

Table 2 Biotin optimization for biliverdin production in C. glutamicum BV004

Biotin concentration (μg/L)	Cell biomass (OD₆₀₀)	Biliverdin (mg/L)	Biliverdin/cell biomass (OD₆₀₀)
1	40.12 ± 0.22	5.17 ± 1.29	0.13
5	49.80 ± 0.63	7.97 ± 0.33	0.16
20	55.41 ± 0.58	9.88 ± 0.38	0.18
50	55.79 ± 1.11	8.66 ± 0.48	0.16
100	54.00 ± 0.92	7.68 ± 0.61	0.14
200	55.10 ± 0.90	11.38 ± 0.47	0.21
300	59.30 ± 0.92	9.79 ± 0.77	0.17
500	58.93 ± 1.91	8.95 ± 1.46	0.15

C. glutamicum BV004 were fermented at 150 rpm for 60 h with 40 g/L initial glucose as a sole carbon source. Results are the means ± standard deviations in three individual experiments

ISSN: 1754-1611