Table 1 Primers used for site-directed mutagenesis
Mutation site | Primers | Sequence 5′-3′ |
|---|---|---|
A138E | F | CATCCAGCCAATAATTCTCCGGACCTGAGAAATCC |
R | GGATTTCTCAGGTCCGGAGAATTATTGGCTGGATG | |
Q147K | F | GAGGGGAAATTTTAAGAGAGCC |
R | GGCTCTCTTAAAATTTCCCCTC | |
I188K | F | GTCCCGGTTTTACCGGATAGTTTTCTGCCATTTGATTCTTCT |
R | AGAAGAATCAAATGGCAGAAAACTATCCGGTAAAACCGGGAC | |
S190Y | F | AAGTCCCGGTTTTACCGTATAG |
R | TCTATACGGTAAAACCGGGAC | |
V197D | F | CTCCGTTGATATCTGAAGTCC |
R | GGGACTTCAGATATCAACGG | |
S19C/G24C | ||
S19C | F | GATGACTGCACCTTTTTTGATGGCT |
R | GGTGCAGTCATCTTCGTATTCTACA | |
G24C | F | TTTGATTGCTTCTCAGGAGGTTTTG |
R | AGAAGCAATCAAAAAAGGTGCAGTC | |
R50C/Q147C | ||
R50C | F | CCGCCTGTTTCGCACCTGCTTCTAC |
R | GCGAAACAGGCGGTGCTTTCTTTCC | |
Q147C | F | TCTCTTTGTATTTCCCCTCTTGAAC |
R | GAAATACAAAGAGAGCCATCCAGCC | |
S76C/ L96C | ||
S76C | F | CAATTGTCAAATGACGTGGGACGGA |
R | CATTTGACAATTGTTCTTCGTGATGATCCC | |
L96C | F | AGGATTGTTTCTCTGCGATGAGCAG |
R | GCAGAGAAACAATCCTGGTCTTGAT | |
S135C/S145C | ||
S135C | F | ATTTCTGTGGTCCGGCGAATTATTG |
R | CGGACCACAGAAATCCTCATTTCCA | |
S145C | F | GATGGCTGTCTTCAAATTTCCCCTC |
R | TGAAGACAGCCATCCAGCCAATAAT | |
E183C/ I188C | ||
E183C | F | TTAGAATGCTCAAATGGCAGAATTC |
R | TTGAGCATTCTAAACGTATCGAATC | |
I188C | F | GGCAGATGTCTATCCGGTAAAACC |
R | ATAGACATCTGCCATTTGAGCATTC | |