|
Drug
|
BlaR-CTD
|
A138E/S19C/G24C
|
Q147K/S19C/G24C
|
I188K/S19C/G24C
|
S190Y/S19C/G24C
|
V197D/S19C/G24C
|
A138E/R50C/Q147C
|
S190Y/R50C/Q147C
|
V197D/R50C/Q147C
|
I188K/R50C/Q147C
|
A138E/E183C/I188C
|
Q147K/E183C/I188C
|
S190Y/E183C/I188C
|
V197D/E183C/I188C
|
|---|
|
Penicillin G
|
23.7 ± 3.5
|
18.5 ± 1.2
|
45.3 ± 4.5
|
7.4 ± 1.3
|
34.0 ± 3.3
|
32.2 ± 4.4
|
7.6 ± 1.0
|
36.5 ± 5.6
|
67.8 ± 7.9
|
10.4 ± 2.0
|
8.4 ± 1.0
|
8.9 ± 0.7
|
7.4 ± 1.1
|
47.1 ± 4.3
|
|
Ampicillin
|
28.2 ± 2.6
|
17.6 ± 3.4
|
5.1 ± 1.1
|
7.5 ± 1.4
|
10.9 ± 1.3
|
16.8 ± 1.7
|
37.3 ± 5.1
|
18.3 ± 2.3
|
10.6 ± 1.2
|
11.2 ± 2.1
|
14.0 ± 2.2
|
6.8 ± 1.1
|
7.2 ± 1.4
|
10.2 ± 1.4
|
|
Amoxicillin
|
21.2 ± 2.4
|
20.5 ± 3.4
|
5.3 ± 0.6
|
12.6 ± 2.4
|
8.8 ± 1.2
|
16.5 ± 1.1
|
11.6 ± 1.3
|
21.6 ± 2.7
|
12.3 ± 1.5
|
11.0 ± 1.3
|
8.6 ± 1..3
|
6.7 ± 0.6
|
7.2 ± 1.0
|
10.0 ± 1.2
|
|
Nafcillin
|
28.9 ± 3.7
|
27.9 ± 4.3
|
14.9 ± 0.7
|
18.4 ± 2.1
|
31.1 ± 2.5
|
14.6 ± 2.3
|
13.1 ± 2.0
|
26.5 ± 3.5
|
12.1 ± 1.2
|
17.8 ± 2.5
|
13.0 ± 2.5
|
9.6 ± 0.7
|
8.3 ± 1.2
|
15.3 ± 1.1
|
|
Oxacillin
|
24.0 ± 1.8
|
22.5 ± 2.3
|
5.9 ± 0.6
|
16.6 ± 2.3
|
10.4 ± 1.3
|
24.5 ± 3.5
|
11.7 ± 2.1
|
28.8 ± 3.4
|
13.5 ± 2.3
|
12.8 ± 1.6
|
9.6 ± 1.0
|
7.2 ± 1.0
|
7.0 ± 1.3
|
26.8 ± 3.5
|
|
Azlocillin
|
19.7 ± 2.5
|
12.0 ± 1.1
|
15.2 ± 1.1
|
11.6 ± 2.4
|
28.6 ± 3.4
|
12.6 ± 1.7
|
19.0 ± 1.3
|
19.7 ± 1.2
|
20.5 ± 3.5
|
8.9 ± 0.7
|
7.4 ± 1.0
|
5.5 ± 1.3
|
5.7 ± 0.9
|
11.2 ± 1.2
|
|
Penicillin V
|
24.7 ± 2.6
|
20.1 ± 1.4
|
5.2 ± 1.2
|
16.3 ± 2.2
|
29.4 ± 2.3
|
26.6 ± 3.4
|
17.9 ± 3.1
|
24.8 ± 4.5
|
12.6 ± 2.3
|
9.6 ± 1.0
|
7.8 ± 0.8
|
5.7 ± 1.0
|
6.0 ± 0.8
|
11.5 ± 2.4
|
|
Piperacillin
|
12.8 ± 3.1
|
24.0 ± 4.2
|
14.4 ± 4.1
|
9.2 ± 1.2
|
17.5 ± 2.3
|
15.1 ± 2.2
|
14.3 ± 1.3
|
12.8 ± 2.3
|
16.3 ± 1.2
|
8.9 ± 1.3
|
7.1 ± 1.4
|
5.6 ± 1.1
|
6.0 ± 1.0
|
12.1 ± 1.2
|
|
Dicloxacillin
|
24.3 ± 4.2
|
26.2 ± 1.3
|
4.8 ± 0.7
|
11.6 ± 2.4
|
29.0 ± 3.2
|
26.6 ± 1.8
|
24.5 ± 5.1
|
23.2 ± 3.5
|
7.0 ± 0.9
|
11.7 ± 2.3
|
9.2 ± 0.6
|
6.7 ± 0.5
|
8.1 ± 0.7
|
21.2 ± 3.4
|
|
Cloxacillin
|
23.6 ± 2.4
|
18.5 ± 2.4
|
15.2 ± 2.3
|
10.2 ± 4.2
|
29.4 ± 2.4
|
28.3 ± 3.4
|
14.2 ± 0.8
|
24.2 ± 2.7
|
24.3 ± 2.3
|
12.0 ± 2.1
|
9.6 ± 1.5
|
7.0 ± 1.2
|
6.7 ± 1.0
|
10.9 ± 1.1
|
|
Cefalotin
|
17.3 ± 1.5
|
13.0 ± 3.4
|
24.8 ± 3.4
|
8.6 ± 0.9
|
9.2 ± 1.1
|
14.3 ± 2.2
|
14.2 ± 1.0
|
8.1 ± 1.0
|
14.8 ± 2.2
|
11.6 ± 2.0
|
9.9 ± 1.1
|
6.7 ± 1.1
|
6.4 ± 1.1
|
38.7 ± 4.7
|
|
Cefoperazone
|
20.3 ± 2.4
|
8.7 ± 1.6
|
9.9 ± 1.1
|
6.2 ± 1.0
|
27.7 ± 3.3
|
18.8 ± 1.1
|
5.9 ± 0.4
|
8.6 ± 0.8
|
7.3 ± 1.1
|
21.6 ± 3.1
|
23.2 ± 3.7
|
7.7 ± 1.4
|
22.5 ± 1.3
|
9.2 ± 1.6
|
|
Cefazolin
|
31.3 ± 4.6
|
15.9 ± 2.3
|
20.8 ± 3.3
|
10.1 ± 2.6
|
21.4 ± 2.6
|
19.6 ± 2.3
|
12.1 ± 3.3
|
47.6 ± 6.7
|
19.1 ± 1.2
|
33.0 ± 2.4
|
21.2 ± 1.8
|
20.7 ± 2.2
|
13.7 ± 2.2
|
19.4 ± 2.2
|
|
Cefalexin
|
80.1 ± 9.7
|
83.3 ± 9.4
|
65.6 ± 7.9
|
63.5 ± 6.7
|
65.9 ± 5.9
|
80.1 ± 7.9
|
79.7 ± 6.4
|
38.4 ± 5.8
|
85.0 ± 9.9
|
91.5 ± 9.4
|
69.8 ± 5.5
|
69.0 ± 7.8
|
66.6 ± 7.9
|
77.4 ± 8.9
|
|
Cefatriaxone
|
22.1 ± 4.6
|
22.5 ± 2.3
|
26.5 ± 3.2
|
10.9 ± 1.0
|
41.9 ± 6.7
|
61.7 ± 7.7
|
15.0 ± 2.5
|
12.6 ± 1.3
|
29.5 ± 3.4
|
37.2 ± 4.6
|
26.6 ± 4.0
|
17.7 ± 2.3
|
10.2 ± 1.5
|
29.2 ± 3.4
|
|
Cefadroxil
|
70.2 ± 5.5
|
63.8 ± 5.4
|
58.8 ± 4.6
|
45.1 ± 6.1
|
56.3 ± 4.5
|
68.3 ± 5.6
|
59.8 ± 6.7
|
29.4 ± 3.5
|
76.0 ± 8.9
|
81.3 ± 7.6
|
51.0 ± 7.6
|
50.4 ± 5.5
|
37.7 ± 4.3
|
66.2 ± 7.8
|
|
Ceftiofur
|
16.1 ± 1.1
|
10.6 ± 1.7
|
15.2 ± 2.1
|
7.6 ± 0.8
|
12.0 ± 1.3
|
26.8 ± 1.5
|
8.8 ± 1.2
|
10.1 ± 1.2
|
14.2 ± 1.2
|
26.3 ± 4.8
|
24.2 ± 2.1
|
16.4 ± 1.6
|
8.3 ± 1.1
|
15.9 ± 3.4
|
|
Cefepime
|
13.2 ± 3.3
|
9.5 ± 1.1
|
13.2 ± 1.3
|
6.0 ± 1.0
|
18.3 ± 1.1
|
13.0 ± 4.7
|
7.1 ± 0.9
|
8.3 ± 1.1
|
8.9 ± 1.0
|
25.2 ± 2.2
|
26.7 ± 3.7
|
20.3 ± 2.2
|
16.6 ± 2.4
|
8.2 ± 0.7
|
|
Cefaclor
|
19.2 ± 2.2
|
32.6 ± 2.3
|
17.1 ± 3.4
|
8.1 ± 1.4
|
28.3 ± 2.7
|
38.4 ± 2.2
|
13.8 ± 2.1
|
13.0 ± 1.3
|
26.8 ± 3.4
|
40.2 ± 4.3
|
36.8 ± 4.6
|
31.4 ± 3.4
|
15.5 ± 2.3
|
28.9 ± 2.3
|
|
Cefquinome
|
21.1 ± 1.4
|
12.6 ± 2.2
|
4.4 ± 1.1
|
6.3 ± 0.7
|
21.5 ± 1.4
|
14.1 ± 1.3
|
6.4 ± 1.0
|
6.5 ± 0.8
|
27.8 ± 2.2
|
25.3 ± 3.4
|
23.1 ± 2.1
|
16.8 ± 1.2
|
16.8 ± 1.6
|
29.0 ± 4.5
|
|
Cefradine
|
59.6 ± 6.9
|
66.5 ± 8.9
|
68.0 ± 7.8
|
43.1 ± 8.7
|
51.8 ± 6.7
|
58.1 ± 8.9
|
74.2 ± 5.9
|
69.1 ± 5.2
|
65.4 ± 8.3
|
74.1 ± 6.4
|
58.7 ± 8.9
|
76.1 ± 7.6
|
74.6 ± 8.6
|
53.3 ± 8.9
|
|
Cefapirin
|
13.5 ± 3.4
|
10.2 ± 2.3
|
5.6 ± 1.1
|
5.8 ± 1.1
|
11.2 ± 1.7
|
14.9 ± 1.1
|
6.4 ± 1.1
|
6.9 ± 0.9
|
9.3 ± 1.4
|
22.2 ± 2.5
|
15.3 ± 2.4
|
16.0 ± 3.3
|
13.2 ± 1.5
|
7.4 ± 1.2
|
|
Cefuroxime
|
44.3 ± 5.6
|
47.4 ± 5.6
|
49.1 ± 6.5
|
30.0 ± 4.5
|
68.9 ± 5.5
|
57.1 ± 4.5
|
36.1 ± 3.5
|
20.5 ± 2.4
|
46.3 ± 3.4
|
39.2 ± 6.7
|
31.4 ± 3.8
|
26.0 ± 2.2
|
29.5 ± 3.6
|
33.1 ± 4.6
|
|
Moxalactam
|
20.0 ± 1.3
|
10.6 ± 1.5
|
14.9 ± 4.2
|
7.4 ± 0.8
|
29.6 ± 4.3
|
21.2 ± 3.4
|
29.0 ± 3.3
|
8.1 ± 1.0
|
12.0 ± 1.1
|
18.8 ± 2.7
|
18.4 ± 4.5
|
16.5 ± 2.7
|
17.9 ± 2.4
|
28.7 ± 3.4
|
|
Cefalonium
|
8.9 ± 1.1
|
7.6 ± 2.1
|
14.4 ± 1.1
|
6.5 ± 1.5
|
30.8 ± 5.4
|
28.0 ± 2.3
|
5.1 ± 1.0
|
6.6 ± 0.8
|
6.5 ± 0.8
|
19.8 ± 3.8
|
19.3 ± 1.2
|
14.8 ± 2.6
|
12.3 ± 3.3
|
9.9 ± 1.5
|
|
Cefminox
|
25.5 ± 3.6
|
42.4 ± 3.8
|
48.7 ± 6.9
|
20.2 ± 3.3
|
67.2 ± 7.5
|
66.9 ± 7.8
|
24.5 ± 4.5
|
17.5 ± 2.4
|
79.0 ± 5.8
|
24.8 ± 3.3
|
17.6 ± 3.3
|
22.5 ± 3.4
|
25.0 ± 4.1
|
82.0 ± 9.2
|
|
Ceftazidime
|
20.2 ± 4.4
|
10.8 ± 1.2
|
15.9 ± 3.4
|
8.1 ± 1.0
|
33.3 ± 3.6
|
37.2 ± 3.2
|
8.6 ± 2.2
|
9.1 ± 1.0
|
14.9 ± 2.2
|
21.7 ± 2.7
|
21.4 ± 3.2
|
17.4 ± 1.2
|
12.0 ± 2.3
|
13.4 ± 1.3
|
|
Carbenicillin
|
8.9 ± 1.1
|
6.1 ± 1.1
|
15.3 ± 4.2
|
6.8 ± 0.8
|
26.6 ± 4.5
|
30.1 ± 4.4
|
6.1 ± 1.1
|
7.4 ± 1.2
|
9.2 ± 1.6
|
10.0 ± 1.9
|
21.3 ± 2.4
|
21.7 ± 3.3
|
8.5 ± 0.9
|
8.1 ± 0.8
|
|
Cefoxitin
|
14.9 ± 2.2
|
9.3 ± 0.7
|
22.9 ± 2.2
|
9.7 ± 1.7
|
52.4 ± 6.7
|
40.6 ± 6.6
|
16.2 ± 3.4
|
12.2 ± 2.3
|
25.0 ± 3.5
|
9.2 ± 2.4
|
9.6 ± 1.0
|
10.2 ± 1.1
|
12.8 ± 2.2
|
14.0 ± 2.4
|
|
Floxacillin
|
6.3 ± 0.8
|
5.4 ± 0.9
|
15.0 ± 1.3
|
4.7 ± 1.3
|
33.8 ± 4.2
|
26.1 ± 6.3
|
5.6 ± 0.8
|
6.6 ± 1.1
|
7.5 ± 1.1
|
17.6 ± 1.3
|
13.1 ± 1.6
|
12.2 ± 2.6
|
6.8 ± 1.4
|
6.9 ± 1.1
|
|
Sulbenicillin
|
18.9 ± 2.3
|
10.4 ± 1.3
|
15.2 ± 2.1
|
7.2 ± 0.8
|
32.9 ± 3.7
|
32.7 ± 2.4
|
8.1 ± 1.1
|
7.9 ± 1.2
|
12.6 ± 2.3
|
20.6 ± 3.2
|
18.7 ± 3.3
|
13.3 ± 3.7
|
14.8 ± 3.3
|
9.3 ± 1.4
|
|
Ticarcillin
|
19.7 ± 1.7
|
10.5 ± 1.5
|
17.3 ± 3.4
|
8.8 ± 1.3
|
36.2 ± 5.4
|
30.9 ± 3.2
|
8.1 ± 1.6
|
8.0 ± 1.0
|
15.4 ± 1.2
|
16.9 ± 2.7
|
15.2 ± 1.2
|
14.8 ± 2.2
|
14.4 ± 2.7
|
10.2 ± 1.0
|
|
Aztreonam
|
102.5 ± 9.1
|
95.7 ± 10.4
|
100.1 ± 9.3
|
90.3 ± 6.6
|
62.5 ± 6.8
|
70.2 ± 6.5
|
105.6 ± 9.1
|
65.5 ± 8.2
|
74.3 ± 8.2
|
102.5 ± 9.9
|
89.6 ± 9.7
|
98.6 ± 7.8
|
95.0 ± 9.3
|
85.0 ± 5.3
|
- Note: The statistical method was used to analyze the significant difference between the wild-type protein and mutant proteins. The underlined number indicated that the inhibition rate was significantly increased, and the boldface number showed that the inhibition rate was significantly reduced (p < 0.05
