Table 1 Sequences and primers for the cloning and expression of genes for the proteins pet28a-Hapo, pet28a-T, pet28a-P, pet28a-L, pet28a-LplA, and pETduet-H-LplA
Primer | Oligonucleotide Sequence | Restriction Site | NCBI NO. |
|---|---|---|---|
ecH-fwd | CATGCCATGGGCAGCAACGTACCAGCAGAACTGAAATAC | NcoI | WP_001295377.1 |
ecH-rev | CCGCTCGAGCTCGTCTTCTAACAATGCTTCGTATGC | XhoI | |
ecT-fwd | CATGCCATGGCACAACAGACTCCTTTGTACGAACAA | NcoI | WP_099356926.1 |
ecT-rev | CCGCTCGAGCGCGACGGCTTTGCCGTTACGCACAAAAAC | XhoI | |
ecP-fwd | CATGCCATGGGCACACAGACGTTAAGCCAGCTTGAAAAC | NcoI | WP_112929453.1 |
ecP-rev | CCGCTCGAGCTGGTATTCGCTAATCGGTACGCAGGAGCAG | XhoI | |
ecL-fwd | CCGCTCGAGTTACTTCTTCTTCGCTTTCGGGTTC | XhoI | WP_110826218.1 |
ecL-rev | GGGAATTCCATATGATGAGTACTGAAATCAAAACTCAGGTCG | NdeI | |
ecLplA-fwd | CCATGGGCTCCACATTACGCCTGCTCATCTCT | NcoI | WP_000105885.1 |
ecLplA-rev | CTCGAGCTACCTTACAGCCCCCGCCAT | XhoI | |
pETduet-ecH-LplA | |||
H-fwd | CATGCCATGGGCAGCAACGTACC | NcoI | |
H-rev | CCCAAGCTTGGCTTTGTTAGCAGCCGGATC | HindIII | |
vector-fwd | GGCCACGCGATCGCTG | Infusion | |
vector-rev | TATCCAATTGAGATCTGCCATATGTATATCTCCTTCTTAT | ||
Fragement-fwd | GATCTCAATTGGATAATGGGCTCCACATTACGCC | ||
Fragement-rev | AGCGATCGCGTGGCCCTACCTTACAGCCCCCGC | ||