Fig. 2

Characterization of confluent monolayer cultures. a Electron microscopy images of the monolayer surfaces covered with microvilli at day 10. Scale Bar = 4 μm. b A cross-section of the monolayers stained for F-actin (green) and integrin β4 (red). Scale bar = 10 μm. c Fluorescence images of the monolayers at day 10 stained with a cell adhesion protein β-Catenin (red) and Hoechst 33342 (nuclei). Scale bar = 10 μm. d Monolayer staining of a tight junction protein, ZO-1 (red), and Hoechst 33342 (nuclei, blue). Scale bar = 10 μm