Fig. 4

Evaluating compound transport across the monolayer platforms. a Experimental design of luminal to basolateral and basolateral to luminal transport assays. b Permeability values of the monolayer on the culture systems to atenolol (paracellular transported drug, low permeability molecule, n = 3) and propranolol (passive transcellular transported drug, high permeability molecule, n = 3). c Permeability of digoxin in the basal to luminal or luminal to basal direction in the two culture formats without (left panel) and with (right panel) the P-gp inhibitor zosuquidar (n = 3). The inhibition data was not significantly different for the four data points. d Permeability of prazosin in the basal to luminal or luminal to basal direction in the two culture formats without (left panel) and with (right panel) the BCRP inhibitor Ko143 (n = 3). The inhibition data was not significantly different for the for the two transport directions on each scaffold