Fig. 5
Investigation of eGFP expression after the co-transfection of fibroblasts with eGFP mRNA and different amounts of B18R mRNA using fluorescence microscopy. 1 × 105 fibroblasts were transfected for two following days with 1.5 μg eGFP mRNA alone or with 0.2, 0.5, 1, or 1.5 μg B18R mRNA. The eGFP expression was analyzed by fluorescence microscopy 24 h after (a) the first and (b) second transfection. Scale bar corresponds to 200 μm