Fig. 6

Impact of co-transfection with synthetic B18R mRNA on cell viability. Fibroblasts were transfected daily up to 7 days with 1.5 μg eGFP mRNA. Additionally, cells were transfected with 1.5 μg eGFP mRNA and 1.5 μg B18R mRNA or incubated with 200 ng/ml B18R protein after the transfection with 1.5 μg eGFP mRNA. The viability of cells after the Lipofectamine^® 2000 treatment was set to 100%. Results are presented as means ± SEM (n = 3). Differences were analyzed using two-way ANOVA following Bonferroni’s multiple comparison test. (*p < 0.05, **p < 0.01, ****p < 0.0001). ns: not significant