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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Contribution of nonconsensus base pairs within ArsR binding sequences toward ArsR-DNA binding and arsenic-mediated transcriptional induction

Fig. 1

Luciferase analysis of reporter constructs containing different combinations of two binding sequences. a: Luciferase activities of pECBS-AFBS and pAFBS-ECBS were measured and compared between the lysates prepared from cells treated with (grey) and without (open) 10 μM arsenite for 1 h. b: Luciferase activities ratios of untreated to arsenic-treated cells were determined. The ratios were compared between pECBS-AFBS and pAFBS-ECBS, pECBS-smt2/1BS and pECBS-arsRBCBS, and psmt2/1BS-AFBS and parsRBSBS-AFBS. C: Sequences of ArsR binding core motif and CS, assigned nucleotides marked with underline. D: Luciferase activities ratios of untreated to arsenic-treated cells were determined. The ratios were compared among pECBS-AFBS, pECBS-CS, pCS-AFBS, pAFBS-CS and parsRBCBS-CS

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Journal of Biological Engineering

ISSN: 1754-1611